Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Chromatin immunoprecipitation (1 paper)Enzyme-linked immunosorbent assay (1 paper)

Goat polyclonal anti-Mouse IgM (µ-chain specific) antibody may be used to identify and quantitate the level of mouse IgM in vitro and in human serum or other biological fluids by immunochemical or immunohistological techniques.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mouse serum-derived IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Reconstitution

Reconstitute with 0.135 M sodium chloride.

Specificity

Goat polyclonal anti-Mouse IgM (µ-chain specific) antibody reacts with the m-chain of mouse IgM. It is s specific for mouse IgM when tested against purified mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM, myeloma proteins.